Showing the last 30 topics from:

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High but variable sequence loss (32-68%) during DADA2 filtering with V3-V4 amplicons (MiSeq 600 cycles)13Wed Apr 01 2026
Problem with cache using qiime cutadapt1049Mon Mar 30 2026
Possible bug in qiime cutadapt demux paired (2024.10)675Wed Jan 28 2026
Q2-cutadapt add "--nextseq-trim" option369Fri Feb 27 2026
Huge difference of DADA2 output with and without removing primers585Tue Jan 27 2026
qiime cutadapt does not have an untrimmed sequences output5116Mon Dec 01 2025
ITS denoising truncation260Wed Oct 15 2025
Duplicate barcode error, but barcodes listed are not the same.790Tue Aug 19 2025
Inconsistent results with discard untrimmed reads in cutadapt7117Thu Jul 10 2025
cutadapt outside of QIIME2155Mon Aug 04 2025
QIIME 2 Meta-Analysis of Rhizosphere Microbiome with Mixed V3-V4/V4 Data: Best Practices for Downstream Analysis14471Thu Apr 17 2025
Analysing V4/V5 16S rRNA illumina Miseq paired-end reads.16366Tue Mar 18 2025
Trimming Paired-End Sequences with cutadapt While Maintaining Pairing Integrity271Mon Feb 10 2025
Which position is best for trimming in a paired-end approach?11142Fri Jan 24 2025
Comparing amplicon studies that use different primers9179Wed Nov 20 2024
Trimming 5bp in QIIME2 2024.5 Amplicon441Thu Nov 14 2024
Error messages after cutadapt, but got trimmed files.5129Thu Nov 07 2024
Cut adapt results in empty files4109Fri Oct 18 2024
QIIME2 Demultiplexing Sequence7199Tue Sep 03 2024
One vs two adaptor sequences using cutadapt499Fri Sep 27 2024
V3-V4 16s - variable size after merging7233Mon Aug 05 2024
problem with cutadapt trim-single6137Tue Jul 02 2024
Error during deblur process8302Sat May 11 2024
Cutadapt - trimming adaptor and primer sequences3222Sat May 11 2024
Missing plugins to load Artifact to run cut adapt2141Tue May 07 2024
Seeking Advice on Primer and Adapter Trimming for MiSeq Amplicon Sequencing Data Using Cutadapt0167Thu Jun 13 2024
Removal of Illumina Adapters1192Thu May 30 2024
Primer show up again after removal using cutadapt in qiime2 DADA211401Mon May 06 2024
I fell into the swamp of 273bp after trimming adapters and primers through cutadapt...8240Mon Mar 18 2024
Pyrosequence work in Qiime2 (Barcode)11276Fri Mar 15 2024