| High but variable sequence loss (32-68%) during DADA2 filtering with V3-V4 amplicons (MiSeq 600 cycles) | | 1 | 3 | Wed Apr 01 2026 |
| denoise failure | | 6 | 56 | Tue Feb 24 2026 |
| High number of unique sequences in PacBio full-length 16S CCS data with DADA2 | | 1 | 39 | Thu Mar 12 2026 |
| Inadequate overlap for V3-V4 (paired-end) due to quality drop | | 5 | 83 | Mon Mar 09 2026 |
| Integrating V3-V4 and V4 16S datasets for large-scale meta-analysis: Is Greengenes2 the optimal choice for cross-region alignment? | | 1 | 87 | Fri Mar 06 2026 |
| Too many elements for DADA2 - denoise in batches? | | 6 | 121 | Wed Mar 04 2026 |
| Analysis with DADA2... Merge Read Length | | 1 | 36 | Wed Mar 04 2026 |
| Huge difference of DADA2 output with and without removing primers | | 5 | 85 | Tue Jan 27 2026 |
| pair ended demultiplexed qza file causing strange error. | | 2 | 53 | Sun Dec 28 2025 |
| High read loss due to artifacts and chimera after denoising 16S V3-V4 paired-end reads | | 2 | 53 | Wed Dec 17 2025 |
| Advice in best practice for handling multiple sequencing runs and subset analyses in QIIME 2 (DADA2) | | 2 | 42 | Fri Jan 16 2026 |
| Error was encountered while running DADA2 in R (return code 1) | | 9 | 92 | Mon Dec 01 2025 |
| Denoising low quality reverse reads in paired-end sequencing reads | | 5 | 72 | Wed Dec 03 2025 |
| Issue with UNITE classifier for ITS1 | | 2 | 61 | Tue Dec 02 2025 |
| DADA2 denoising taking long time | | 7 | 88 | Mon Dec 01 2025 |
| Questions about performing quality control with dada2 and generating a feature table | | 3 | 41 | Sat Nov 29 2025 |
| QIIME2 non-chimeric reads | | 4 | 71 | Mon Nov 10 2025 |
| Significant 16S sequence loss while merging w/ DADA2, various truncation settings used | | 3 | 115 | Wed Nov 05 2025 |
| DADA2 Fails on AVITI Data | | 8 | 159 | Wed Oct 29 2025 |
| qiime dada2 denoise-paired is unresponsive | | 3 | 62 | Mon Oct 27 2025 |
| DADA2 priors trimming | | 1 | 30 | Tue Nov 18 2025 |
| priors in qiime2-dada2 | | 4 | 251 | Tue Nov 18 2025 |
| Observed ASV richness drops after adding 3rd MiSeq library — why do I lose ~100 species when I merge libraries? | | 4 | 36 | Thu Oct 16 2025 |
| ITS denoising truncation | | 2 | 60 | Wed Oct 15 2025 |
| Need help choosing truncation lengths for DADA2 and interpreting the demultiplexed sequence count summary. | | 2 | 47 | Mon Oct 13 2025 |
| DADA2 -- denoise | | 2 | 62 | Fri Nov 07 2025 |
| Interpreting KEGG vs MetaCyc pathways in 16S (PICRUSt2) functional prediction | | 1 | 111 | Sun Oct 19 2025 |
| Converting 16S V3–V4 ASV reads to absolute copy numbers (qPCR / spike-in calibration?) | | 3 | 100 | Thu Oct 09 2025 |
| Chimera removal dilemma in 16S V3–V4: necessary when using Kraken2/Bracken? | | 8 | 154 | Fri Sep 26 2025 |
| Why is non-chimera read count often referred to as sequencing depth in 16S rRNA analysis? | | 4 | 152 | Thu Sep 18 2025 |
